Abstract: Tumor necrosis factor-alpha (TNF-) could be considered as potential biomarkers in atopic dermatitis (AD), while its level could be influenced by cytokine single gene polymorphisms (SNP). This study was performed in 89 pediatric patients with AD and 137 controls to assess polymorphisms of the TNF- gene at positions -308 and -238, using the polymerase chain reaction and the sequence-specific primers method. The highest positive allelic association that made the patients susceptible to AD was seen for TNF- -238/G (p<0.001) and TNF- -308/G (p = 0.003). The GG genotypes at TNF- -238 and TNF- -308, were both significantly higher in the patients with AD, compared to the controls (p<0.01). The GG haplotype at TNF- (-308,-238) was seen in 92.7% of the patients, which was significantly higher than the controls (p<0.001), while a negative haplotypic association withADwas seen for TNF- (-308, -238) AG and GA (p<0.01). This study showed that the AG genotype of TNF- -308, associated with a high production of cytokines, was significantly decreased in patients with AD, while the low-producing GG genotype, which could lead to low production of TNF-, was over-expressed in the atopic patients. Keywords: atopic dermatitis, cytokine, single gene polymorphisms, tumor necrosis factor-alpha